Dandan Huang

Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China
Author    Correspondence author
Bioscience Methods, 2025, Vol. 16, No. 5   doi: 10.5376/bm.2025.16.0024
Received: 06 Aug., 2025    Accepted: 17 Sep., 2025    Published: 09 Oct., 2025

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Huang D.D., 2025, Optimizing irrigation and fertilization regimes to enhance yield and quality in kiwifruit, Bioscience Methods, 16(5): 254-261 (doi: 10.5376/bm.2025.16.0024)

As a sustainable agricultural input, green manure has attracted wide attention in the application of legume rotation system in recent years. This study systematically analyzed the application effect of green manure in legume rotation, aiming to clarify its comprehensive impact on crop yield, soil fertility and microbial community, and improve the ecological service function of the rotation system. Through case studies, the performance of different types of green manure (such as astragalus, vetch, and oat) in rotation was compared, focusing on the nitrogen supply capacity, changes in soil organic matter content, and the response of rhizosphere microbial diversity. The results showed that the rational application of green manure not only significantly increased the yield of legume crops, but also improved the physical and chemical properties of the soil, and promoted the enrichment of microbial diversity and beneficial communities. This study provides a theoretical basis and practical guidance for promoting the efficient use of green manure in legume rotation, and promoting the construction of a green agricultural system.

Green manure; Legume rotation; Soil fertility; Microbial community; Nitrogen supply